Web56°C in 80 µL of proteinase K with 5 mL of Buffer RLT from the Rneasy Midi kit and then processed per manufac-turer’s directions. RT-PCR RT-PCR for WNV was performed on the tissues according to the procedure described by Kuno (6) and using the RT-nested primer sets described by Johnson et al. (7). In brief, a RT-PCR mixture was prepared by ...
Tumor cell invasion into Matrigel: optimized protocol for RNA ...
Web* Maximum binding capacity of RNeasy mini spin column is 100 µg of RNA. * Buffer RLT may form precipitate upon storage. If necessary, warm to redissolve. * Add 10 μl ß-ME per … WebBuffer RLT Plus* 45 ml 220 ml ... The RNeasy Plus Mini Kit should be stored dry at room temperature (15–25°C) and is stable for at least 9 months under these conditions, if not … food that produces energy
Mutation-induced infections of phage-plasmids Nature …
WebRNeasy® Mini QIAcube® Kit For use on QIAcube Connect and classic QIAcube instruments. RNeasy Mini QIAcube Kit (240) Cat. no. 74116 RNeasy Mini Spin Columns 240 Rotor Adapters 240 Elution Tubes 240 Buffer RLT* 220 ml Buffer RW1* 220 ml Buffer RPE† (concentrate) 65 ml RNase-Free Water 50 ml Product sheet 1 * Contains a guanidine salt. WebOct 12, 2005 · RLT buffer. Four mls of ß-ME/RLT is required for each sample. 2. Tissue is quickly weighed (>20-150 mg) and placed in a medium-sized weigh boat containing ß-ME/RLT buffer (1 ml) and minced using 2 sterile razor blades for tissue disruption. 3. Pour the remaining ß-ME/RLT (3 ml) in a 15 ml round-bottom polypropylene tube (not provided). Web(Kontes/VWR; IL) in RLT buffer (Qiagen; RNeasy Mini Kit; Alameda, CA), centrifuged at 4°C, and stored at −80°C until RNA isolation. RNA extraction and cDNA synthesis RNA extraction from pupal thoracic tissue was done with reagents from the RNeasy Mini Kit and RNase-Free DNase Set (Qiagen; Alameda, CA) with the following modifica-tions. food that people eat on christmas